Bartholomeus Abrahamsz. Assteyn (Dordrecht 1607, probably Dordrecht 1669/1677) was a Dutch painter. Bartholomeus Assteyn was the son of a painter from Ghent, Abraham Bartholomeusz. In the year 1631 he became member in the Dordrecht’s Saint Lukas painter gild. Assteyn lived with his family in Vriesestraat. In 1651 he was registered as an accountant for the local painter brotherhood. His exact death date is not known; his last authenticated work dates from the year 1669. Assteyn was a productive artist. Although he is not considered to be innovative, he created noteworthy still lifes. Assteyn’s compositions are particularly affected by the Johannes Bosschaert, and his painting technique is reminiscent of Balthasar van der Ast.